Immunofluorescence and Tissue Culture

Rabies antibody determination by immunofluorescence in tissue culture.

A tissue culture (TC)-fluorescent antibody (FA) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. This test was performed with BHK-21 cells infected with the ERA vaccine virus strain on Lab-Tek TC chamber slides. A Flury high egg passage (HEP) rabies virus strain grown on continuous line of Afri...

Quenching autofluorescence in tissue immunofluorescence

Background: Immunofluorescence (IF) is one of the most important techniques where fluorochromes conjugated to antibodies are used to detect specific proteins or antigens. In tissue sections, autofluorescence (AF) can lead to poor quality images that impair assessment. The placenta is a pivotal extra-embryonic organ in embryo development, where trophoblasts make up a large proportion of the cell...

Storage of virus-infected tissue culture substrates for the detection of serum antibodies by immunofluorescence.

Multiparameter immunofluorescence on paraffin-embedded tissue sections.

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...

Immunofluorescence and cytochemical studies of visna virus in cell culture.

Sequential morphological changes occurring in sheep choroid plexus cells infected with visna virus were studied by direct immunofluorescence, acridine orange, and hematoxylin and eosin staining methods. Specific immunofluorescence was first detected in the perinuclear cytoplasm of solitary cells 24 hr after infection. As the infection progressed, viral antigen appeared in an increasing number o...